Andrea Sinz

Andrea Sinz

received a degree in Pharmacy from the University of Tübingen, Germany, in 1993. She obtained her PhD in Pharmaceutical Chemistry from the University of Marburg, Germany, in 1997. From 1998-2000, she was a post-doctoral fellow at the National Institutes of Health in Bethesda, MD, USA, where she became introduced into chemical cross-linking techniques and protein mass spectrometry. After research stays at the Universities of Gießen and Rostock, she became head of a junior research group at the University of Leipzig, Germany (2001-2006). Since 2007, she is Full Professor and head of the Department of Pharmaceutical Chemistry and Bioanalytics at the MLU. She is an expert in chemical cross-linking and mass spectrometry for studying protein conformations and protein-protein interactions. Her research interests are the development of novel analytical strategies and reagents to advance the cross-linking/MS approach. Since 2017, she is president of the German Society for Mass Spectrometry (DGMS). She is the spokesperson of the RTG 2467.

Project within the RTG

Protein interaction partners of the tumor suppressor p53 will be studied by chemical cross-linking/mass spectrometry. After the covalent fixation of protein binding partners, the p53 complexes are purified, digested and the peptides are analyzed by mass spectrometry.

Key publications

Iacobucci C, Götze M, Ihling CH, Arlt C, Hage C, Schmidt R, Piotrowski C, Sinz A. A cross-linking/mass spectrometry workflow based on MS-cleavable cross-linkers and the MeroX software for studying protein structures and protein-protein interactions. Nat Protocols. 2018, 12: 2864-2889.

Hage C, Iacobucci C, Rehkamp A, Arlt C, Sinz A. The first zero-length mass spectrometry-cleavable cross-linker for protein structure analysis. Angew Chem Int Ed Engl. 2017, 56: 14551-14555.

Arlt C, Flegler V, Ihling CH, Schäfer M, Thondorf I, Sinz A. An integrated mass spectrometry based approach to probe the structure of the full-length wild-type tetrameric p53 tumor suppressor. Angew Chem Int Ed Engl. 2017, 56: 275-279.